Recombinant 2019-nCoV Methyltransferase / ME Protein (His Tag) | 763-PKSR030513

Recombinant 2019-nCoV Methyltransferase / ME Protein (His Tag) | PKSR030513 | Elabscience.

Synonym: Methyltransferase, ME

Activity: N/A

Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Methyltransferase / ME (YP_009724389.1) (Ser6799-Asn7096) was expressed with a polyhistidine tag at the C-terminus.

Sequence: Ser6799-Asn7096

Fusion tag: C-6His

Accession: YP_009724390.1

Species: SARS-CoV-2

Expressed Host: E.coli

Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass: 33.46kDa.

AP Mol Mass: N/A

Formulation: Lyophilized from sterile 20 mM Tris 150 mM NaCl, pH 8.3.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Coronavirus encodes the 2'-O-MTase（2'O Methyltransferase） that is composed of the catalytic subunit nsp16 and the stimulatory subunit nsp10 and plays an important role in virus genome replication and evasion from innate immunity during viral infection. Nonstructural protein 16 (NSP16) / viral 2'O-methyltransferase (2'O-MTase) is highly conserved. The conserved 2'O-MTase activity is important for CoV pathogenesis and NSP16 is a conserved universal target for rapid live attenuated vaccine design in an expanding Coronavirus outbreak setting, such as COVID-19. Targeting on the 2'O-methylation pathway on SARS-CoV replication and pathogenesis can be the treatment options for vaccine and anti-viral durgs development which can against SARS-CoV-2，SARS-CoV, MERS-CoV or other RNA and DNA viruses.