Recombinant 2019-nCoV NSP8 Protein (His Tag) | 763-PKSR030470
- IN STOCK
Recombinant 2019-nCoV NSP8 Protein (His Tag) | PKSR030470 | Elabscience.
Synonym: SARS-CoV 2 nsp8
Protein Construction: Recombinant 2019-nCoV NSP8 is produced by our E.coli expression system and the target gene encoding Ala1-Gln198 is expressed with a 6His tag at the C-terminus.
Fusion tag: C-6His
Expressed Host: E.coli
Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Mol Mass: 25 kDa
AP Mol Mass: 25 kDa
Formulation: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.5.
Reconstitution: Not Applicable
Background: Cleavage by the viral main protease, 3CLpro results in generating the nsp8 protein, The nsp8 protein has been shown to associate with several other nsps and to colocalize with these nsps in cytoplasmic complexes that are important for viral RNA synthesis. It forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. Nsp8 was shown to have RNA-dependent RNA polymerase (RdRp) activity that could be involved in producing primers utilized by nsp12 which is normally accepted to be the RdRp for SARS-CoV.