Recombinant 2019-nCoV NSP7 Protein (His Tag) | 763-PKSR030469

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Recombinant 2019-nCoV NSP7 Protein (His Tag) | PKSR030469 | Elabscience.

Synonym: SARS-CoV 2 nsp7

Activity: N/A

Protein Construction: Recombinant 2019-nCoV NSP7 is produced by our E.coli expression system and the target gene encoding Ser1-Gln83 is expressed with a 6His tag at the C-terminus.

Sequence: Ser1-Gln83

Fusion tag: C-6His

Accession: YP_009725303.1

Species: SARS-CoV-2

Expressed Host: E.coli

Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass: 12.3 kDa

AP Mol Mass: 12 kDa

Formulation: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.5.

Reconstitution: Not Applicable

Background: The ∼30kb positive-stranded RNA genome of coronaviruses encodes a replication/transcription machinery that is unusually complex and composed of 16 nonstructural proteins (nsps) . The four proteins nsp7 to nsp10, which are conserved among all CoVs but have no functional homologs outside of the Coronaviridae, are translated as part of the viral polyproteins pp1a and pp1ab, and the mature proteins are released by the action of the SARS-CoV protease nsp5. Hexadecamer of nsp7 and nsp8 may possess dsRNA-binding activity. SARS-CoV 2 nonstructural protein 7 (nsp7) is of interest for its potential roles in the transcription and replication of the positive-stranded viral RNA genome.

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