Recombinant 2019-nCoV Envelope Protein (His Tag) | 763-PKSR030488

Recombinant 2019-nCoV Envelope Protein (His Tag) | PKSR030488 | Elabscience.

Synonym: 2019-nCoV E protein; 2019-nCoV sM protein

Activity: N/A

Protein Construction: Recombinant 2019-nCoV Envelope Protein is produced by our E.coli expression system and the target gene encoding Met1-Val75 is expressed with a BBP, 6His tag at the N-terminus.

Sequence: Met1-Val75

Fusion tag: N-6His

Accession: QHD43418.1

Species: SARS-CoV-2

Expressed Host: E.coli

Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass: 27.5 kDa

AP Mol Mass: 22-25 kDa

Formulation: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 200 mM NaCl, pH 8.0.

Reconstitution: Not Applicable

Background: Coronavirus envelope (E) proteins are short (100 residues) polypeptides that contain at least one transmembrane (TM) domain and a cluster of 2-3 juxtamembrane cysteines. These proteins are involved in viral morphogenesis and tropism, and their absence leads in some cases to aberrant virions, or to viral attenuation. In common to other viroporins, coronavirus envelope proteins increase membrane permeability to ions, plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis. Activates the host NLRP3 inflammasome, leading to IL-1beta overproduction.