SARS-CoV-2 S1 protein NTD (L18F, T20N, P26S, D138Y, R190S), His Tag (MALS verified) | S1D-C52He

SARS-CoV-2 S1 protein NTD (L18F, T20N, P26S, D138Y, R190S), His Tag (MALS verified) | S1D-C52He | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

S1 protein NTD, Spike protein S1 NTD, BetaCoV S1-NTD

Source

SARS-CoV-2 S1 protein NTD, His Tag (S1D-C52He) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1). The L18F/ T20N/ P26S/ D138Y/ R190S mutations were identified on the spike protein N-terminal domain (NTD) in the SARS-CoV-2 variant (known as P.1) which emerged in Brazil.
Predicted N-terminus: Ser 13

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 34.9 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.