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- SARS-CoV-2 S1 protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y), His Tag | S1N-C52Hp
Acro Biosystems
SARS-CoV-2 S1 protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y), His Tag | S1N-C52Hp
- SKU:
- S1N-C52Hp
- UPC:
- MPN:
- Host:
- HEK293
- Lineage:
- SARS-CoV-2 Gamma | P.1 (Brazil)
- Molecule:
- Spike S1
- Mutation:
- L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y
- Species:
- SARS-CoV-2
- Tag:
- His Tag
Description
Synonym
Spike, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1
Source
SARS-CoV-2 S1 protein, His Tag (S1N-C52Hp) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y mutations were identified in the SARS-CoV-2 Gamma variant (Pango lineage: P.1; other names: 20J/501Y.V3).
Predicted N-terminus: Val 16
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
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