SARS-CoV-2 Nucleocapsid protein (P13L, R203K, G204R, G214C), His Tag | NUN-C52Ha

SARS-CoV-2 Nucleocapsid protein (P13L, R203K, G204R, G214C), His Tag | NUN-C52Ha | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

Nucleocapsid protein, NP, Protein N

Source

SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C52Ha) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). Mutations P13L, R203K, G204R, G214C were identified in the SARS-CoV-2 Lambda variant (Pango lineage: C.37).
Predicted N-terminus: Met 1

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, 1mM PMSF, pH 7.3. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.