Recombinant SARS-CoV-2 Spike S1 (D614G) (His Tag) (Active) | 763-PKSV030387

Recombinant SARS-CoV-2 Spike S1 (D614G) (His Tag) (Active) | PKSV030387 | Elabscience.

Synonym: S1 protein; 2019-nCoV S1 protein; coronavirus S1 Protein; cov S1 Protein

Activity: Immobilized Recombinant Human ACE-2 Protein (Fc Tag) (Active) (Cat#PKSR030492) at 5μg/ml (100 μl/well) can bind Recombinant 2019-nCoV S1 Protein (His Tag) (D614G) (Active) (Cat#PKSV030387) .The ED50 of PKSV030387 is 1.43 μg/ml.

Protein Construction: Recombinant 2019-nCoV S1 Protein is produced by our Mammalian expression system and the target gene encoding Gln14-Arg685 (D614G) is expressed with a 10His tag at the C-terminus.

Sequence: Gln14-Arg685 (D614G)

Fusion tag: C-10His

Accession: QHD43416.1

Species: SARS-CoV-2

Expressed Host: Human Cells

Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass: 76.6kDa

AP Mol Mass: 100-130kDa

Formulation: Supplied as a 0.2 μM filtered solution of PBS, pH 7.4.

Reconstitution: Not Applicable

Background: The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS) . The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD) , which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.