Recombinant SARS-CoV-2 Spike S1+S2 ECD (D614G) (His Tag) | 763-PKSV030392

Recombinant SARS-CoV-2 Spike S1+S2 ECD (D614G) (His Tag) | PKSV030392 | Elabscience.

Synonym: N/A

Activity: Measured by its binding ability in a functional ELISA.Immobilized human ACE2 protein (Fc tag) (PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (D614G) -His (PKSV030392) , the EC50 of PKSV030392 is 0.5-1.2 μg/mL.

Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) (YP_009724390.1) (Met1-Pro1213 (D614G) ) was expressed with a polyhistidine tag at the C-terminus.

Sequence: Met1-Pro1213

Fusion tag: C-His

Accession: YP_009724390.1

Species: SARS-CoV-2

Expressed Host: Baculovirus-Insect Cells

Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by SDS-PAGE

Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass: 134.3 kDa

AP Mol Mass: N/A

Formulation: Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.

Reconstitution: Please refer to the printed manual for detailed information.

Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD) , which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD) , leading to a higher mortality rate.