Recombinant SARS-CoV-2 N Protein biotinylated (N-6His-Avi) (Active) | 763-PKSV030311

Recombinant SARS-CoV-2 N Protein biotinylated (N-6His-Avi) (Active) | PKSV030311 | Elabscience.

Synonym: SARS-CoV-2 coronavirus NP Protein; SARS-CoV-2 np Protein; SARS-CoV-2 novel coronavirus

Activity: Immobilized 2019-nCoV NP Antibody (6G9) at 5μg/ml (100 μl/well) can bind 2019-nCoV Nucleocapsid Protein-His-Avi (PKSV030311) . The ED50 of PKSV030311 is 92 ng/ml.

Protein Construction: Biotinylated 2019-nCoV Nucleocapsid Protein is produced by our E.coli expression system and the target gene encoding Met1-Ala419 is expressed with a 6His, Avi tag at the N-terminus.

Sequence: Met1-Ala419

Fusion tag: N-6His

Accession: QHD43423.2

Species: SARS-CoV-2

Expressed Host: E.coli

Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass: 51.3kDa

AP Mol Mass: 55-60kDa

Formulation: Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH 7.5

Reconstitution: Not Applicable

Background: Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.