Recombinant 2019-nCoV Nucleocapsid Protein, Biotinylated (His Tag) | 763-PKSR030512

Recombinant 2019-nCoV Nucleocapsid Protein, Biotinylated (His Tag) | PKSR030512 | Elabscience.

Synonym: NP;2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein

Activity: N/A

Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (YP_009724397.2) (Met1-Ala419 (335Gly/Ala) ) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.

Sequence: (Met1-Ala419 (335Gly/Ala) )

Fusion tag: C-6His

Accession: YP_009724397.2

Species: SARS-CoV-2

Expressed Host: Baculovirus-Insect Cells

Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass: 47.08 kDa

AP Mol Mass: N/A

Formulation: Lyophilized from sterile PBS, 500 mM NaCl, pH 7.0.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.