Recombinant 2019-nCoV Nucleocapsid Protein, Biotinylated (His Tag) | 763-PKSR030512
- IN STOCK
- 20 µg
Recombinant 2019-nCoV Nucleocapsid Protein, Biotinylated (His Tag) | PKSR030512 | Elabscience.
Synonym: NP;2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (YP_009724397.2) (Met1-Ala419 (335Gly/Ala) ) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Sequence: (Met1-Ala419 (335Gly/Ala) )
Fusion tag: C-6His
Expressed Host: Baculovirus-Insect Cells
Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 85 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Mol Mass: 47.08 kDa
AP Mol Mass: N/A
Formulation: Lyophilized from sterile PBS, 500 mM NaCl, pH 7.0.
Reconstitution: Please refer to the printed manual for detailed information.
Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.