Recombinant 2019-nCoV NSP10 Protein (His Tag) | 763-PKSR030471

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Recombinant 2019-nCoV NSP10 Protein (His Tag) | PKSR030471 | Elabscience.

Synonym: SARS-CoV 2 nsp10; SARS-CoV 2 Growth factor-like peptide; SARS-CoV 2 GFL

Activity: N/A

Protein Construction: Recombinant 2019-nCoV NSP10 is produced by our E.coli expression system and the target gene encoding Ala1-Gln139 is expressed with a 6His tag at the N-terminus.

Sequence: Ala1-Gln139

Fusion tag: N-6His

Accession: YP_009725306.1

Species: SARS-CoV-2

Expressed Host: E.coli

Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.

Purity: > 80 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass: 17.9 kDa

AP Mol Mass: 18 kDa

Formulation: Supplied as a 0.2 μM filtered solution of PBS, 10% Glycerol, pH 7.4.

Reconstitution: Not Applicable

Background: Nsp10 have shown that it is a 15-kDa protein of unknown function that has been shown to interact with itself, nsp1, and nsp7. It colocalizes with N to sites of viral replication and is essential for replication. It plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation. Nsp10 is a critical regulator of coronavirus RNA synthesis and may play an important role in polyprotein processing.

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