HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (MALS verified) | SPN-H52H5

HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (MALS verified) | SPN-H52H5 | Acro Biosystems | SARS-CoV-2 Other Coronavirus Proteins

Synonym

Spike, S protein, Spike glycoprotein, S glycoprotein

Source

HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (SPN-H52H5) is the ectodomain of HCoV-HKU1 (isolate N5) spike protein which contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. The S1/S2 furin-recognition site 752-RRKRR-756 is mutated to GGSGS to generate the uncleaved protein. Proline substitutions (N1067P, L1068P) are introduced to stabilize the trimeric prefusion state of the spike protein.
Predicted N-terminus: Ala 13

Molecular Characterization

Online(Ala 13 - Asn 1276) Q0ZME7-1Development Suggestion
This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 145.5 kDa. The protein migrates as 180-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution