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- HCoV-NL63 Spike Trimer, His Tag | SPN-H52H4
Acro Biosystems
HCoV-NL63 Spike Trimer, His Tag | SPN-H52H4
- SKU:
- SPN-H52H4
- UPC:
- MPN:
- Host:
- HEK293
- Molecule:
- Spike protein
- Species:
- HCoV-NL63
- Tag:
- His Tag
Description
HCoV-NL63 Spike Trimer, His Tag | SPN-H52H4 | Acro Biosystems | SARS-CoV-2 Other Coronavirus Proteins
Synonym
Spike, S protein, Spike glycoprotein, S glycoprotein
Source
HCoV-NL63 Spike Trimer, His Tag (SPN-H52H4) is expressed from human 293 cells (HEK293). It contains Phe 16 - Asn 1291 (Accession # Q6Q1S2-
1).The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the N-terminus.
Predicted N-terminus: Phe 16
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 146.9 kDa. The protein migrates as 200-240 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
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