HCoV-229E Spike Trimer, His Tag | SPN-H52H3
- Spike protein
- His Tag
HCoV-229E Spike Trimer, His Tag | SPN-H52H3 | Acro Biosystems | SARS-CoV-2 Other Coronavirus Proteins
Spike, S protein, Spike glycoprotein, S glycoprotein
HCoV-229E Spike Protein, His Tag (SPN-H52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 16 - Pro 1115 (Accession # P15423-1 (TI 871-872 PP)).The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the N-terminus. Proline substitutions TI 871-872 PP are introduced to stabilize the trimeric prefusion state of the spike protein.
Predicted N-terminus: Cys 16
Online(Cys 16 - Pro 1115) P15423-1Development Suggestion
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 125.7 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.