Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His, Avitag™ | SPD-C82Ef
- SARS-CoV-2 B.1.620 (Africa/Europe)
- Spike RBD
- S477N, E484K
- His Tag & Avi Tag
Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His, Avitag™ | SPD-C82Ef | Acro Biosystems | SARS-CoV-2 Protein Mutants
Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag (SPD-C82Ef) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1(S477N, E484K)). The mutations (S477N, E484K) were identified in the SARS-CoV-2 variants which emerged in Europe (known as B.1.620).
Predicted N-terminus: Arg 319
Online(Arg 319 - Lys 537) QHD43416.1 (S477N, E484K)Development Suggestion
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 28.2 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.