Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His, Avitag™ | SPD-C82Ef

Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His, Avitag™ | SPD-C82Ef | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

Source

Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag (SPD-C82Ef) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1(S477N, E484K)). The mutations (S477N, E484K) were identified in the SARS-CoV-2 variants which emerged in Europe (known as B.1.620).
Predicted N-terminus: Arg 319

Molecular Characterization

Online(Arg 319 - Lys 537) QHD43416.1 (S477N, E484K)Development Suggestion
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 28.2 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.