Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His, Avitag™ | S1N-C82E3

Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His, Avitag™ | S1N-C82E3 | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

Spike, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1

Source

Biotinylated SARS-CoV-2 S1 protein (D614G), His,Avitag™ (S1N-C82E3) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1(D614G)).
Predicted N-terminus: Val 16

Molecular Characterization

Online(Val 16 - Arg 685) QHD43416.1 (D614G)Development Suggestion
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 78.5 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution