Acro Biosystems
Biotinylated SARS-CoV-2 (COVID-19) S2 protein, His, Avitag™ | S2N-C52E8
- SKU:
- S2N-C52E8
- UPC:
- MPN:
- Host:
- HEK293
- Molecule:
- Spike S2
- Species:
- SARS-CoV-2
- Tag:
- His Tag & Avi Tag
Description
Biotinylated SARS-CoV-2 (COVID-19) S2 protein, His, Avitag™ | S2N-C52E8 | Acro Biosystems | SARS-CoV-2 Proteins
Synonym
Spike, S2 protein, Spike glycoprotein Subunit2, S glycoprotein Subunit2, Spike protein S2
Source
Biotinylated SARS-CoV-2 S2 protein, His,Avitag™ (S2N-C52E8) is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1).
Predicted N-terminus: Ser 686
Molecular Characterization
Online(Ser 686 - Pro 1213) QHD43416.1Development Suggestion
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 61.5 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution
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