SARS S protein (R667A, K968P, V969P), His Tag (MALS verified) | SPN-S52H6

SARS S protein (R667A, K968P, V969P), His Tag (MALS verified) | SPN-S52H6 | Acro Biosystems | SARS-CoV-2 Other Coronavirus Proteins

Synonym

Spike, S protein, Spike glycoprotein, S glycoprotein

Source

SARS S protein (R667A, K968P, V969P), His Tag (SPN-S52H6) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1(R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Ser 14

Molecular Characterization

Online(Ser 14 - Pro 1195) AAP13567.1 (R667A, K968P, V969P)Development Suggestion
This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.