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- SARS-CoV-2 Spike Trimer (T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N), His Tag | SPN-C52Ht
Acro Biosystems
SARS-CoV-2 Spike Trimer (T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N), His Tag | SPN-C52Ht
- SKU:
- SPN-C52Ht
- UPC:
- MPN:
- Host:
- HEK293
- Lineage:
- SARS-CoV-2 Delta Plus | AY.1 | AY.2 | AY.3 (India)
- Molecule:
- Spike Trimer
- Mutation:
- T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N
- Species:
- SARS-CoV-2
- Tag:
- His Tag
Description
SARS-CoV-2 Spike Trimer (T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N), His Tag | SPN-C52Ht | Acro Biosystems | SARS-CoV-2 Protein Mutants
Synonym
Spike, S protein, Spike glycoprotein, S glycoprotein
Source
SARS-CoV-2 Spike Trimer, His Tag (SPN-C52Ht) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). Mutations T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N were identified in the SARS-CoV-2 Delta sub-lineages (Pango lineage: AY.1/AY.2/AY.3; also known as "Delta Plus"). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Val 16
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 137.8 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
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