SARS-CoV-2 Spike S2-coupled Magnetic Beads | MBS-K018

The SARS-CoV-2 Spike S2-coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike S2 protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.

 

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

 

See Certificate of Analysis (CoA) for detailed instruction.

 

Assay Principles

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

Antibody Purification:

1. Resuspend the lyophilized beads by adding the buffer of choice.

2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.

3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.

4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.