SARS-CoV-2 Spike RBD (L452R, T478K, N501Y), His Tag | SPD-C5225
- SARS-CoV-2 (Vietnam)
- Spike RBD
- L452R, T478K, N501Y
- His Tag
SARS-CoV-2 Spike RBD (L452R, T478K, N501Y), His Tag | SPD-C5225 | Acro Biosystems | SARS-CoV-2 Protein Mutants
Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
SARS-CoV-2 Spike RBD (L452R, T478K, N501Y), His Tag (SPD-C5225) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1(L452R, T478K, N501Y)). The mutations (L452R, T478K, N501Y) were identified in the SARS-CoV-2 variants which emerged in Vietnam.
Predicted N-terminus: Arg 319
Online(Arg 319 - Lys 537) QHD43416.1 (L452R, T478K, N501Y)Development Suggestion
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.