SARS-CoV-2 Spike RBD (L452R, E484Q), Fc Tag (MALS verified) | SPD-C525d

SARS-CoV-2 Spike RBD (L452R, E484Q), Fc Tag (MALS verified) | SPD-C525d | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

Source

SARS-CoV-2 Spike RBD (L452R, E484Q), Fc Tag (SPD-C525d) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1(L452R, E484Q)). The mutations (L452R, E484Q) were identified in the SARS-CoV-2 variants which emerged in India (known as B.1.617).
Predicted N-terminus: Arg 319

Molecular Characterization

Online(Arg 319 - Lys 537) QHD43416.1 (L452R, E484Q)Development Suggestion
This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 51.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.