Acro Biosystems
SARS-CoV-2 Spike RBD (L452Q, F490S), His Tag | SPD-C5227
- SKU:
- SPD-C5227
- UPC:
- MPN:
- Host:
- HEK293
- Lineage:
- SARS-CoV-2 Lambda | C.37 (South America)
- Molecule:
- Spike RBD
- Mutation:
- L452Q, F490S
- Species:
- SARS-CoV-2
- Tag:
- His Tag
Description
SARS-CoV-2 Spike RBD (L452Q, F490S), His Tag | SPD-C5227 | Acro Biosystems | SARS-CoV-2 Protein Mutants
Synonym
Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
Source
SARS-CoV-2 Spike RBD, His Tag (SPD-C5227) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1(L452Q, F490S)). Mutations L452Q, F490S are identified in the SARS-CoV-2 Lambda Variant (C.37).
Predicted N-terminus: Arg 319
Molecular Characterization
Online(Arg 319 - Lys 537) QHD43416.1 (L452Q, F490S)Development Suggestion
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
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