SARS-CoV-2 Spike Protein IgM ELISA Kit | E-EL-E603
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SARS-CoV-2 Spike Protein IgM ELISA Kit | E-EL-E603 | Elabscience
Target Species: Human
Assay Time: 1.5h
Detection Type: Colormetric
Detection Range: N/A
Target Name: SARS-CoV-2 Spike Protein IgM
Target Synonym: COVID 19 IgM, S protein IgM
Tested Sample Types: Serum and plasma
Test Principle: This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Spike protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Spike protein antigen, after adding samples to wells, the SARS-CoV-2 Spike protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Spike protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike protein IgM exists in the tested samples or not.
Specificity: This kit recognizes Human SARS-CoV-2 Spike Protein IgM in samples. No significant cross-reactivity or interference between Human SARS-CoV-2 Spike Protein IgM and analogues was observed.
Application: This ELISA kit applies to the in vitro qualitative determination of Human SARS-CoV-2 Spike Protein IgM concentrations in Serum and plasma.
Research Areas: SARS-CoV-2, COVID 19
Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.