SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag (MALS verified) | S1D-C52Hf

SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag (MALS verified) | S1D-C52Hf | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

S1 protein NTD, Spike protein S1 NTD, BetaCoV S1-NTD

Source

SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag (S1D-C52Hf) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1(T95I, G142D, E154K)). The mutations (T95I, G142D, E154K) were identified in the SARS-CoV-2 variants which emerged in India (known as B.1.617).
Predicted N-terminus: Ser 13

Molecular Characterization

Online(Ser 13 - Leu 303) QHD43416.1 (T95I, G142D, E154K)Development Suggestion
This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 35.0 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.