SARS-CoV-2 S protein (F817P, A892P, A899P, A942P, K986P, V987P), His Tag (MALS verified) | SPN-C52Hd

SARS-CoV-2 S protein (F817P, A892P, A899P, A942P, K986P, V987P), His Tag (MALS verified) | SPN-C52Hd | Acro Biosystems | SARS-CoV-2 Proteins

Synonym

Spike, S protein, Spike glycoprotein, S glycoprotein

Source

SARS-CoV-2 S protein, His Tag (SPN-C52Hd) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein.
Predicted N-terminus: Val 16

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 138.2 kDa. The protein migrates as 75-85 kDa, 90-100 kDa and 150-160 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution