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SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified) | SPN-C52H3

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SKU:
SPN-C52H3
Host:
HEK293
Lineage:
SARS-CoV-2 (D614G)
Molecule:
Spike protein
Mutation:
D614G
Species:
SARS-CoV-2
Tag:
His Tag
zł2,454.88 - zł14,078.92
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Description

SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified) | SPN-C52H3 | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym
 
Spike, S protein, Spike glycoprotein, S glycoprotein
 
Source
 
SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (SPN-C52H3) is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1) and D614G mutation, which has become increasingly common in SARS-CoV-2 viruses from around the world . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
 
Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 138.0 kDa. The protein migrates as 170-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin
 
Less than 1.0 EU per μg by the LAL method.
 
Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution

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