SARS-CoV-2 Nucleocapsid protein (S194L), His Tag | NUN-C52He

SARS-CoV-2 Nucleocapsid protein (S194L), His Tag | NUN-C52He | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

Nucleocapsid protein, NP, Protein N

Source

SARS-CoV-2 Nucleocapsid protein (S194L), His Tag (NUN-C52He) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1(S194L)). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. Phosphorylation of this SR-link motif in SARS-CoV modulates nucleocapsid multimerization, translational inhibitory activity and cellular localization. The mutation S194L was identified on the SR-linker on the nucleocapsid protein of SARS-CoV-2 variants with a global frequency of about 3.2% and high frequency in the variants circulating in the UK, the US and India (data retrieved from https://pubmed.ncbi.nlm.nih.gov/33095454/).
Predicted N-terminus: Met 1

Molecular Characterization

Online(Met 1 - Ala 419) QHO62115.1 (S194L)Development Suggestion
This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.