SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag | NUN-C52Hg

SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag | NUN-C52Hg | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

Nucleocapsid protein, NP, Protein N

Source

SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag (NUN-C52Hg) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (R203K, G204R)). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutations (R203K, G204R) were identified on the SR-linker on the nucleocapsid protein of SARS-CoV-2 variants. Phosphorylation of this SR-link motif in SARS-CoV modulates nucleocapsid multimerization, translational inhibitory activity and cellular localization. The co-occuring mutations R203K and G204R may decrease the overall structural flexibility of SARS-COV-2 N protein.
Predicted N-terminus: Met 1

Molecular Characterization

Online(Met 1 - Ala 419) QHO62115.1 (R203K, G204R)Development Suggestion
This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.