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SARS-CoV-2 Nucleocapsid protein (D63G, R203M, G215C, D377Y), His Tag B.1.1.7 (U.K.) | NUN-C52Hr

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SKU:
NUN-C52Hr-1
Host:
HEK293
Lineage:
SARS-CoV-2 Delta | B.1.1.7 (U.K.)
Molecule:
Nucleocapsid protein
Mutation:
D63G, R203M, D377Y, R390K
Species:
SARS-CoV-2
Tag:
His Tag
€482.00 - €2,858.00
Frequently bought together:

Description

SARS-CoV-2 Nucleocapsid protein (D63G, R203M, G215C, D377Y), His Tag B.1.1.7 (U.K.) | NUN-C52Hr | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym
 
Nucleocapsid protein, NP, Protein N
 
Source
 
SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C52Hr) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). The mutations (D63G, R203M, G215C, D377Y) were identified in the SARS-CoV-2 Delta lineages (Pango lineage: B.1.617.2; GISAID clade: 21A, 21I, 21J; Nextstrain clade: G/478K.V1).
Predicted N-terminus: Met 1
 
Molecular Characterization
 

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.
 
Purity
 
>95% as determined by SDS-PAGE.
 
Formulation
 

Lyophilized from 0.22 μm filtered solution in PBS,pH7.3 with 0.2 M Arginine. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution
 

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
 

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

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