SARS-CoV-2 (B.1.1.7) Spike RBD-coupled Magnetic Beads | MBS-K034

The SARS-CoV-2 Spike RBD (B.1.1.7) Coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike RBD to streptavidin-conjugated magnetic beads. The spike RBD coupled to the beads contains the critical N501Y mutation identified in multiple circulating SARS-CoV-2 variants, e.g. the U.K. variant (known as B.1.1.7, 20B/501Y.V1 or VOC 202012/01), the South African variant (known as B.1.351 or 20C/501Y.V2) and the Brazilian variant (known as P.1). The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.

 

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

 

See Certificate of Analysis (CoA) for detailed instruction.

 

Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

 

Antibody Purification:

1. Resuspend the lyophilized beads by adding the buffer of choice.

2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.

3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.

4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.