Recombinant SARS-CoV-2 S Protein RBD (C-6His) V2 (Active) | 763-PKSV030308

Recombinant SARS-CoV-2 S Protein RBD (C-6His) V2 (Active) | PKSV030308 | Elabscience.

Synonym: SARS-CoV-2 RBD Protein; SARS-CoV-2 Spike RBD Protein

Activity: Immobilized 2019-nCoV S Protein RBD-His V2 (PKSV030308) at 5μg/ml (100 μl/well) can bind Human ACE-2-Fc (PKSR030492) .The ED50 of Recombinant Human ACE-2-Fc (PKSR030492) is 417 ng/ml；Immobilized 2019-nCoV S Protein RBD-His V2 (PKSV030308) at 5μg/ml (100 μl/well) can bind Anti-2019-nCoV S1 mAb (5D9) .The ED50 of Anti-2019-nCoV S1 mAb (5D9) is 117 ng/ml.

Protein Construction: Recombinant 2019-nCoV S protein RBD Protein is produced by our Mammalian expression system and the target gene encoding Arg319-Phe541 is expressed with a 6His tag at the C-terminus.

Sequence: Arg319-Phe541

Fusion tag: C-6His

Accession: QHD43416.1

Species: SARS-CoV-2

Expressed Host: Human Cells

Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass: 25.9kDa

AP Mol Mass: 35kDa

Formulation: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Reconstitution: Not Applicable

Background: The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS) . The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD) , which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.