Recombinant 2019-nCoV Nucleoprotein / NP Protein (His Tag) | 763-PKSR030506

Recombinant 2019-nCoV Nucleoprotein / NP Protein (His Tag) | PKSR030506 | Elabscience.

Synonym: 2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein

Activity: N/A

Protein Construction: A DNA sequence encoding the SARS-CoV (isolate:Tor2) nucleoprotein (NP_828858.1) (Met1-Ala422) was expressed with a C-terminal polyhistidine tag.

Sequence: Met1-Ala422

Fusion tag: C-6His

Accession: NP_828858.1

Species: SARS-CoV-2

Expressed Host: Baculovirus-Insect Cells

Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 80 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass: 47.5 kDa

AP Mol Mass: 47.1 kDa

Formulation: LyopHilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.