Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit | RAS-T018 | Acro Biosystems | SARS-CoV-2 Antibody Titer Assay Kits
Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide. The SARS-CoV-2 pandemic presents an extraordinary challenge to the lives and health of the global population, resulting in a total of over 200 million diagnosed cases and 4.9 million death (as of October 2021). It also significantly affected the social, economic and environmental domains throughout the world and changed everyone’s life. ACROBiosystems, a leading life science company dedicated to aid the global COVID-19 response，developed different kits suitable for serum antibody detection in different species (Mouse, Monkeys, humans) to meet the needs of preclinical and clinical experiments.
This kit is developed for serologic test for Mouse IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
After reconstitution, the stock solution should be kept at -70°C.
It is recommended not to freeze thaw more than 3 times.
This product is stable under storage conditions: 12 months in sealed state;
Used within1 months after opening.
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Spike RBD. The Kit consists of Pre-coated with SARS-CoV-2 Spike RBD Microplate, an Anti-SARS-CoV-2 Antibody (Control, Mouse IgG1), an HRP-Anti-Goat anti mouse IgG secondary antibody.
Your experiment will include 4 simple steps:
a) Add your sample to the plate, take theAnti-SARS-CoV-2 Antibody (Control, Mouse IgG )as Control sample. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.