MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (MALS verified) | SPN-M52H4

MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (MALS verified) | SPN-M52H4 | Acro Biosystems | SARS-CoV-2 Other Coronavirus Proteins

Synonym

Spike, S protein, Spike glycoprotein, S glycoprotein

Source

MERS Spike protein trimer, His Tag (SPN-M52H4) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Trp 1295 (Accession # K9N5Q8-1). The recombinant protein has a T4 fibritin trimerization motif. Proline substitutions (V1060P, L1061P) and alanine substitutions (R748A, R751A) are introduced to stabilize the trimeric prefusion state of MERS-CoV Spike protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Tyr 18

Molecular Characterization

Online(Tyr 18 - Trp 1295) K9N5Q8-1Development Suggestion
This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 145.9 kDa. The protein migrates as 150 kDa and 175-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.