MERS Nucleocapsid protein, His Tag | NUN-M52H5

MERS Nucleocapsid protein, His Tag | NUN-M52H5 | Acro Biosystems | SARS-CoV-2 Other Coronavirus Proteins

Synonym

Nucleocapsid protein, NP, Protein N

Source

MERS Nucleocapsid protein, His Tag (NUN-M52H5) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Asp 413 (Accession # K0BVN3-1).
Predicted N-terminus: Met 1

Molecular Characterization

Online(Met 1 - Asp 413) K0BVN3-1Development Suggestion
This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin cleavage site, 251-RHKR-254, and is partially processed into N and C-terminal fragment with calculated MW of 17.1 kDa and 19.7 kDa respectively. The protein migrates as 23-25 kDa (C-terminus) and 46-73 kDa (full length) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.