HCoV-NL63 Spike Trimer, His Tag | SPN-H52H4

HCoV-NL63 Spike Trimer, His Tag | SPN-H52H4 | Acro Biosystems | SARS-CoV-2 Other Coronavirus Proteins

Synonym

Spike, S protein, Spike glycoprotein, S glycoprotein

Source

HCoV-NL63 Spike Trimer, His Tag (SPN-H52H4) is expressed from human 293 cells (HEK293). It contains Phe 16 - Asn 1291 (Accession # Q6Q1S2-

1).The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the N-terminus.
Predicted N-terminus: Phe 16

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 146.9 kDa. The protein migrates as 200-240 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.