Biotinylated SARS-CoV-2 Spike Trimer (G75V, T76I, SYLTPGD247-253del, L452Q, F490S, D614G, T859N), His, Avitag™ | SPN-C82Eh

Biotinylated SARS-CoV-2 Spike Trimer (G75V, T76I, SYLTPGD247-253del, L452Q, F490S, D614G, T859N), His, Avitag™ | SPN-C82Eh | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

Spike, S protein, Spike glycoprotein, S glycoprotein

Source

Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (SPN-C82Eh) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). Mutations G75V, T76I, SYLTPGD 247-253 del, L452Q, F490S, D614G, T859N were identified in the SARS-CoV-2 Lambda variant (Pango lineage: C.37). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Val 16

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 139.0 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.