Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His, Avitag™ (MALS verified) | SPD-C82Ed

(No reviews yet) Write a Review
SKU:
SPD-C82Ed
Host:
HEK293
Lineage:
SARS-CoV-2 Delta | B.1.617.2 (India)
Molecule:
Spike RBD
Mutation:
L452R, T478K
Species:
SARS-CoV-2
Tag:
His Tag & Avi Tag
Request Pricing: info@gentaur.com
Frequently bought together:

Description

Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His, Avitag™ (MALS verified) | SPD-C82Ed | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym

Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

Source

Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His,Avitag (SPD-C82Ed) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1(L452R, T478K)). The mutations L452R, T478K were identified in the SARS-CoV-2 Delta variant (Pango lineage: B.1.617.2; other names: 21A/S:478K).
Predicted N-terminus: Arg 319

Molecular Characterization

Online(Arg 319 - Lys 537) QHD43416.1 (L452R, T478K)Development Suggestion
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 28.3 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

View AllClose