Biotinylated SARS-CoV-2 S1 protein NTD, His, Avitag™ (MALS verified) | S1D-C52E2

Biotinylated SARS-CoV-2 S1 protein NTD, His, Avitag™ (MALS verified) | S1D-C52E2 | Acro Biosystems | SARS-CoV-2 Proteins

Synonym

S1 protein NTD, Spike protein S1 NTD, BetaCoV S1-NTD

Source

Biotinylated SARS-CoV-2 S1 protein NTD, His,Avitag (S1D-C52E2) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Phe 318 (Accession # QHD43416.1).
Predicted N-terminus: Val 16

Molecular Characterization

Online(Val 16 - Phe 318) QHD43416.1Development Suggestion
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 38.0 kDa. The protein migrates as 53-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.