Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc, Avitag™(MALS verified) | SPD-C82A9

Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc, Avitag™(MALS verified) | SPD-C82A9 | Acro Biosystems | SARS-CoV-2 Proteins

Synonym

Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

Source

Biotinylated SARS-CoV-2 S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) (SPD-C82Aa) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1).
Predicted N-terminus: Arg 319

Molecular Characterization

Online(Arg 319 - Lys 537) QHD43416.1Development Suggestion
This protein carries a mouse IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 52.5 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution