Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His, Avitag™ (MALS verified) | SPN-H82E3

Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His, Avitag™ (MALS verified) | SPN-H82E3 | Acro Biosystems | SARS-CoV-2 Other Coronavirus Proteins

Synonym

Spike, S protein, Spike glycoprotein, S glycoprotein

Source

Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His,Avitag (SPN-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1). Amino acid substitutions RRKRR 752-756 GGSGS and NL 1067-1068 PP are introduced to abolish the furin cleavage site and stabilize the trimeric prefusion state of the spike protein, respectively.
Predicted N-terminus: Ala 13

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

The protein has a calculated MW of 147.2 kDa. The protein migrates as 200-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.