Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD) | RAS-T009

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.

 

This kit is developed for serologic test for Total Anti-SARS-CoV-2 antibody in serum/plasma in vitro.

It is for research use only.

 

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

 

The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

It is recommended not to freeze thaw more than 3 times for powder.

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of total anti-SARS-CoV-2 antibody in serum by SARS-CoV-2 Spike protein RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control, an HRP-Spike RBD and related buffer.

Your experiment will include 4 simple steps:

a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

b) Add diluted HRP-SARS-CoV-2 Spike RBD to the plate. The HRP-SARS-CoV-2 Spike RBD is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.