Anti-SARS-CoV-2 (P.1) Antibody IgG Titer Serologic Assay Kit (Spike RBD) | RAS-T032

 

Multiple variants of SARS-CoV-2 are circulating globally and posting new challenges to human health. The variant P.1 identified in Brazil in Jan, 2021 shows increased transmissibility and is likely to escape from established immunity. There are 12 mutations on the spike protein of the Brazilian variant (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F); of which 3 (K417T, E484K, N501Y) resides on the receptor-binding domain (RBD). To evaluate the impacts of the new variants, a rapid and effective assay kit detecting the levels of antibody against the mutant is in urgent need.

 

This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 Spike RBD antibody in serum/plasma in vitro. The Spike RBD antigen used in this kit contains K417T, E484K, N501Y mutations found in the Brazilian variant (known as P.1).

 

It is for research use only.

 

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

The unopened kit should be stored at 2°C to 8°C. The shelf life is 1 year from the date of receipt. The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.

It is recommended not to freeze thaw more than 3 times.

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control, an HRP-Anti-Human IgG secondary antibody and related buffer.

Your experiment will include 4 simple steps:

a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.