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Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay Kit (Spike Protein) | RAS-T027

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SKU:
RAS-T027
Category:
Antibody titer assay kit
Sample:
Human Serum
Species:
SARS-CoV-2 Alpha | B.1.1.7 (U.K.)
Size:
96 Tests
€752.00
Frequently bought together:

Description

Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay Kit (Spike Protein) | RAS-T027 | Acro Biosystems | SARS-CoV-2 Antibody Titer Assay Kits

Background
 
Multiple variants of SARS-CoV-2 are circulating globally and posting new challenges to human health. The variant B.1.1.7 identified in the United Kingdom (UK) carries a large number of mutations and is reported to spread more easily and quickly than other variants. There are 9 mutations on the spike protein of the U.K. variant: HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H. To evaluate the impacts of the new variants, a rapid and effective assay kit detecting the levels of antibody against the mutant is in urgent need.
 
Application
 
This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 Spike antibody in serum/plasma in vitro. The Spike antigen used in this kit contains 9 mutations (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) found in the U.K. variant (known as B.1.1.7, 20B/501Y.V1 or VOC 202012/01).
 
It is for research use only.
 
Storage

The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

Assay Principles
 
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike Protein. The kit consists of Pre-coated SARS-CoV-2 Spike Protein Microplate, an Positive Control, an Negative Control, an HRP-Anti-Human IgG secondary antibody and related buffer.

Your experiment will include 4 simple steps:

a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

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