Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD) | RAS-T024

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SKU:
RAS-T024
Category:
Antibody titer assay kit
Sample:
Human Serum
Species:
SARS-CoV-2 Wild Type
Size:
96 Tests
zł3,096.08
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Description

Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD) | RAS-T024 | Acro Biosystems | SARS-CoV-2 Antibody Titer Assay Kits

Background
 
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective Assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
 
Application
 
This kit is developed for titer measurement of Anti-SARS-CoV-2 Antibody IgG (Spike RBD) in human serum.
It is for research use only.
 
Reconstitution
 
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
 
Storage

The unopened kit should be stored at 2°C to 8°C. The shelf life is 1 year from the date of receipt. The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.

Assay Principles
 
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control,an HRP-Anti-Human IgG secondary antibody and related buffer.

Your experiment will include 4 simple steps:

a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.

b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

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