SARS-CoV-2 Spike S1 (T95I, G142D, E154K, L452R, E484Q, D614G, P681R), His Tag | S1N-C52Ht

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SKU:
S1N-C52Ht
Host:
HEK293
Lineage:
SARS-CoV-2 Kappa | B.1.617.1 (India)
Molecule:
Spike S1
Mutation:
T95I, G142D, E154K, L452R, E484Q, D614G, P681R
Species:
SARS-CoV-2
Tag:
His Tag
£395.60 - £2,401.98
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Description

SARS-CoV-2 Spike S1 (T95I, G142D, E154K, L452R, E484Q, D614G, P681R), His Tag | S1N-C52Ht | Acro Biosystems | SARS-CoV-2 Protein Mutants

Synonym
 
Spike, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1
 
Source
 
SARS-CoV-2 Spike S1, His Tag (S1N-C52Ht) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). The mutations (T95I, G142D, E154K, L452R, E484Q, D614G, P681R) were identified in the SARS-CoV-2 Kappa variant (Pango lineage: B.1.617.1; other names: 21A/S:154K).
Predicted N-terminus: Val 16
 
Molecular Characterization
 

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 77.0 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.
 
Purity
 

>95% as determined by reduced SDS-PAGE.

Formulation
 

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution
 

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
 

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

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